Composite

Part:BBa_K3329067

Designed by: Wenfei Xiao   Group: iGEM20_BNDS_China   (2020-10-20)


SpyTag003*3-Scl2*2-sfGFP for E. coli

SpyTag003 is a sub part in the Spytag-catcher003 system. It should be used with Spycatcher003. The super-folder GFP protein is connected with scl2. The green color indicates whether the scl2 is bound to cellulose by seeing whether that filter paper turned green.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 198
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1564
    Illegal NgoMIV site found at 2076
    Illegal NgoMIV site found at 2277
    Illegal AgeI site found at 535
    Illegal AgeI site found at 1411
    Illegal AgeI site found at 1420
    Illegal AgeI site found at 1492
    Illegal AgeI site found at 1546
    Illegal AgeI site found at 1762
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 151
    Illegal BsaI site found at 907
    Illegal BsaI site found at 1963
    Illegal BsaI.rc site found at 532


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Categories
Parameters
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